E. coli Well WaterTest Kit Well Water Testing made easy. E. coli test media Quantitubes(TM)) with ID Labels Pipette Large Alcohol Wipes Graduated Cup Incubator box and stand
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Water testing test for e.coli,
identification of e.coli, coliform bacteria test, coliform bacteria
testing, bacteria testing kit test for e. coli, coliform test
kits and coliform bacteria tests.
Test Kit Contents 10 Test
- 10 units E. coli Quantitube(TM) Medium
- 10 E. coli Quantitube(TM) Strips
- 10 Quantitubes(TM)) with ID Lables
- 10 Pipettes
- Graduated Cups
- Large Alcohol Wipes
- Chain of Custody
- Incubator Box/Quantitube(TM) Stand
- Rapid Identification E. coli Bacteria Test
- Rapid Identification E. coli Bacteria Test High-Sensitivity 5ml
- Rapid Identification E. coli Bacteria Test 16 Hour
- Rapid Identification E. coli Bacteria Test Kit
- Rapid Identification E. coli Bacteria Test Quantitube
Rapid Identification 24 hour E. coli and Coliform Coliscan® media- Rapid Identification 24 hour E. coli Bacteria and Coliform Coliscan® media Quantitube
Rapid Identification 16 hour E. coli/Salmonella ECA Check®
| Easygel® Coliscan® Click to order | Easygel® Coliscan® media Quantitube®. | Easygel® E. coli/Coliform Quantitube(TM) |
Micrology Laboratories just rewrote the book on water testing for E. coli, Quantitube® solid culture in a test tube. Overnight results reliably culture on the road.
New from Micrology Laboratories - Easygel® in a Test Tube
Quantitube(TM) is so easy to use.
Quantitube®The media turns solid in the Quantitube®. Snap seal capped test tube means no Petri dish to tape; it's easier to handle and takes less storage room. E.coli Quantitube(TM) 16 hour recovery routine.
Our Water Sample Collection Station
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- 20 E. coli Quantitube(TM) Medium
- 20 E. coli Quantitube(TM) Strips
- 20 Quantitubes(TM)) with ID Lables
- 20 Pipettes
- Graduated Cups
- Large Alcohol Wipes
- Chain of Custody
- Incubator Box/Quantitube(TM) Stand
Laboratory
PackagingInstructions
This is a High-Sensitivity 5 ml water or liquid/soft food E. coli test.
The stand holes for the Quantitube(TM) are under the label of the storage box . Using a Quantitube(TM), push through the label to open the stand hole; there are 5 holes.
Number the Quantitubes(TM) you are going to use for your sample and fill in the Chain of Custody form. The tape can be removed for counting and inspection and then reapplied.
Sampling and measurements: The E. coli Quantitube(TM) Test requires a 5 ml liquid sample. For your convince, there are two way that you can measure your sample: the graduated Quantitube(TM) or the graduated cups.
Field water samples may be collected in the capable graduated Quantitube(TM) .
Samples may be measured in the graduated Quantitube(TM) or the graduated cups included in your kit.
5 ml of any liquid may be added to the Quantitube(TM) . One bottle of E. coli Quantitube(TM) Medium should then be added to the Quantitube(TM) . The cap should be pressed on firmly and the Quantitube(TM) inverted three times. Do not shake; gently invert the Quantitube(TM) three times.
Remove the cap of the Quantitube(TM) and insert the a Quantitube(TM) Strip narrow end first into the Quantitube(TM) . Reseal the Quantitube(TM) and insert into the box stand for incubation.
Using the graduated cup may be easier for some people. Squeeze the cup to form a pour spout. Measure your sample into the cup. Pour the sample into the E. coli Quantitube(TM) Medium. Reseal the bottle. Gently invert the Quantitube(TM) Medium three times. Remove the cap from a Quantitube(TM) . Insert the a Quantitube(TM) into the Box 1 stand. Now pour the contents of the E. coli Quantitube(TM) Medium with the 5 ml sample into the Quantitube(TM) . Insert the a Quantitube(TM) Strip narrow end first into the Quantitube(TM) . Reseal the Quantitube(TM) .
Why This Test Works
E. coli Quantitube(TM)
Medium -- This medium indicates and identifies the presence of
E. coli in a test sample. The E. coli form small teal green colonies
with a teal halo, while other members of the Enterobacteriaceae
grow as colorless colonies. For optimum results, the medium should
be incubated at a temperature of 35 C, and results counted at
24-48 hrs. The teal green color is specific for the enzyme glucuronidase,
which is virtually exclusively found in E. coli among the coliforms.
| Coliscan ® MF Method | m-TEC Method |
| 1. Dispense 2 mL Coliscan broth into each 60 mm dish so that the sterile pad is soaked. | 1. Prepare m-TEC agar dishes. |
| 2. Filter test sample and place filter on the surface of the pad containing the medium. Replace the lid. | 2. Filter test sample and place the filteron the surface of the agar medium. |
| 3. Invert the dish and place in a 35ºC ±0.5 incubator for 24 hrs ±2. | 3. Invert the dish and place in a 35ºC ±0.5 incubator for 2 hours. |
| 4.Remove from incubator and count the blue/purple colonies as E. coli. | 4. Remove from the incubator, seal in a water-tight bag and incubate an additional 22-24 hours at 44.5ºC ±0.2 in a water bath incubator. |
| 5. Remove from the water bath, open the dish and transfer the membrane filter containing colonies which have grown to a dish with a pad soaked by urease reagent (which must be prepared fresh) for several minutes. | |
| 6. Count yellow-brown CFU as E. coli. |
Advantages of the Coliscan ® MF method are numerous.
1. There is no initial 2 hour resuscitation period requiring operator attention and a change of incubators, as well as the placement of the plates in waterproof bags for the water bath incubator.
2. Temperature is not critical. 24 hr incubation at 35ºC ±0.5 is the only requirement. Less time is used in checking and calibrating incubators and no switching from incubator to incubator is needed. (Also, much less expensive incubators can be used.
3. There is no need to do the additional urease test required by the m-TEC method. This saves time and expense. Also, some bacterial types (eg. Pseudomonas spp. ) can result in gross false positive results with the m-TEC method.
4. Coliscan® MF broth is easier to use and plates are easier to prepare than m-TEC agar medium, which must be mixed, sterilized and dispensed into plates, and then stored until use. The Coliscan® MF broth is kept frozen (with a 6 month shelf life) until needed and then the thawed broth is dispensed aseptically into plates containing sterile absorbent pads and fresh medium is assured.
5. Coliscan® MF broth is provided in safe-to-use plastic bottles (20 mL for 10 tests/bottle) which are recyclable. There are no dangerous-to-handle glass vials to break open.
6. Coliscan® MF medium is based upon well proven technology which incorporates chromogenic enzyme substrates. It is superior to the modified m-TEC medium developed by the EPA laboratories which uses only one chromogenic substrate (for the enzyme glucuronidase) and requires the resuscitation period followed by elevated incubation temperature.
With the modified m-TEC medium, it is stated that E. coli is indicated by magenta colored colonies. This is generally true, but it is possible for some temperature tolerant, glucuronidase positive organisms to exist, which could result in false positive readings. Also, if other (non-magenta) colonies grow on the modified m-TEC medium, they should be classified as "fecal" coliforms. However, there is no surety that these non-colored colonies are in fact true coliforms as they may not be galactosidase positive, and the modified m-TEC medium does not test for galactosidase.
The Coliscan® MF medium avoids the potential problems described above by the incorporation of two different chromogenic substrates, one for the enzyme glucuronidase (X-gluc which colors positive colonies a teal green color), and one for the enzyme galactosidase (Red-gal® which colors positive colonies a pink color). Since E. coli is unique among the coliforms as a producer of glucuronidase, it utilizes the X-gluc substrate, causing the expression of the teal green color. However, since E. coli is a true coliform, it also produces galactosidase and so utilizes the second substrate also and the combination of the teal green and the pink colors results in E. coli colonies having a dark blue/purple color. If there are any non-E. coli coliform organisms in the sample, they appear as pink galactosidase positive colonies, while non-coliform, galactosidase negative organisms are colorless.
7. It should be pointed out that if an operator wishes to determine both E. coli and "fecal coliforms", the Coliscan® MF medium is well prepared to accomplish this task. All that is needed is to initiate the same procedure for incubation as that called for with the modified m-TEC method. The 44.5ºC incubation temperature will eliminate non-temperature tolerant organisms so that the E. coli colonies will be blue/purple, but other temperature tolerant coliforms will be pink, and any temperature tolerant non-coliforms will be colorless. This versatility allows the operator to distinguish temperature tolerant "fecal coliforms" which sometimes occur (commonly members of the genus Klebsiella from the more important E. coli.
